常微分方程"一题多解"的启迪

阐述了变量代换法和积分因子法求解一阶非线方程和由积分因子求通积分的方法。     

脂肪酶基因结构和氨基酸序列的比较

采用PCGENE6 8软件系统对真菌脂肪酶基因和氨基酸序列进行了分析。真核生物中多数结构基因中含有内含子 ,但真菌脂肪酶基因几乎有一大半的是连续的 ;对于有内含子的基因 ,不同脂肪酶基因所含内含子数目各异。所有脂肪酶一级结构中都包含Gly X1 Ser X2 Gly保守序列 ,在真菌脂肪酶中该序列更保守 ;Ser、Asp/Glu和His3个氨基酸残基组成了真菌脂肪酶的活性中心 ;大多数真菌脂肪酶是糖蛋白 ,但也有一些不含糖基的脂肪酶 ,主要取决于脂肪酶一级结构中是否存在糖基化识别序列。     

黄河鮈Cytb基因克隆及其在鮈属中的系统发育

采用聚合酶链式反应克隆黄河鮈线粒体细胞色素b基因 (Cyt b),首次报道了该基因的全长序列(1140bp,FJ904648) ,并与鮈属中其他6个物种的Cyt b 基因进行同源性比较,分析了碱基组成和变异情况,以马口鱼属的马口鱼和鯝属的云南鯝作为外群构建了ML和NJ系统发育树.遗传距离和分子系统学分析表明,黄河鮈与犬首鮈具有较低的遗传距离(6.30%),在系统发育树上两者聚在一起,提示黄河鮈与犬首鮈存在较近的亲缘关系.     

淋巴细胞白血病患者CD3基因表达模式的变化

目的:了解T细胞受体信号传导分子CD3γδεζ基因在T和B细胞白血病病人中的表达特点.方法:采用SYBR Green Ⅰ荧光定量PCR和相对定量分析法检测32例淋巴细胞肿瘤病人:急性B淋巴细胞白血病(B-ALL)12例、慢性B淋巴细胞白血病(B-CLL)9例、急性T淋巴细胞白血病(T-ALL)11例及10例健康人外周血...     

Apple品牌产品形态知觉设计之浅析

本文以Apple典型产品形态设计为例,分析产品形态知觉的特征、形态知觉的基本原型、基本原型的基因特征。从而提取出产品出形态信息中的基本原型,构建基于形态知觉的产品造型设计方法。     

Identification of susceptibility genes loci associated with type 2 diabetes

In this study, we selected 10 susceptible SNPs loci to investigate their contribution to susceptibility to type 2 diabetes in Han Chinese among Hubei population. We genotyped SNPs rs5219, rs1801282, rs1470579, rs1111875, rs1081661, rs7754840, rs4506565, rs13266634, rs4402960, and rs5643981 by using the method of polymerase chain reaction-ligase detection reaction (PCR-LDR). In a case-control study, we have genotyped the 10 candidate susceptibility SNP loci, and here, we reported that the SNP rs5219 in KCNJ1...     

体育运动中的基因兴奋剂

WADA将基因或细胞兴奋剂定义为“能增强运动能力的基因，遗传元素和／或细胞的非治疗性的使用”．遗传学和基因学的新的研究结果不仅将用于疾病的诊断和治疗，而且还将用于试图增强机体的运动能力．用于治疗疾病的基因疗法，如贫血（EPO基因）、肌肉营养不良（IGF—I基因）和外周血管疾病（血管内皮生长因子基因）等，都可能被用于兴奋剂．为保护运动员的健康和保证公平竞争，IOC、WADA和ISF（国际体育联合会）已将提高运动能力的药物和方法定义为基因兴奋剂，并禁止其使用．     

Effect of P15INK4b expression on the cell cycle and G1 phase related regulatory genes in human melanoma cells

Using the transfeetion teehnique. P15INK4b was introduced into P15INk4b gene deleted human melanoma A375 cells,and a cell model MLED6 overexpressing P15INK4b WAS CONSTRUCTED.Comparing with the control cells MLC2,MLEK6cells in G1phase increased by 11%,but those in Sphase decreased by 15%by FCM.By the method of thymidine(TdR)and N2O arresting,the proportions of synchronized Mphase cells of MLEK6 ana MLC23 were measured and found to be 89.1% and 76.8%respectively ,and the cells in G1phase were 74.3% for MLID6 AND 76. 4% forMLC2.The result of3 H-TdR incorporation indicated that the transition of G1/Sof MLEK6 cell was delayed 2h as compared with that of MLC2 cells,and incorporation rate also decreased.The observation on exprissions of some G1/ S-resates relatory rigusating genes showed that in MLIK6 cells the protein leves of P27KIPI increased with the decreasing expressions of cyclinD1,cyclinE and c-myc,especially cyclinD1 in late G1phade.The expression of cyclinE obviously decreased at G1/S transition ,and c-myc wad inhibited throughout all the process of G1 S phase.All the risults suggest that P15INK4b can delayG1/S transition of MLEK6 cells by inhibiting the cell cycle engine ,and by increasing the expression of Cdk ingibitor P27KIPI in different stages of G1 phase.